Brief topical and intraluminal use of Carolina rinse solution does not attenuate experimental ischemia and reperfusion injury in rabbit jejunum

ABSTRACT Fifteen New Zealand adult rabbits were randomly allocated into three groups: Sham-operated (group A), Ischemia and Reperfusion (group B) and Carolina Rinse Solution (CRS) (group C). Groups B and C were subjected to one hour of ischemia and two hours of reperfusion. In group C, ten minutes before reperfusion, the bowel lumen was filled with CRS, and the segment immersed in CRS. Necrosis and loss of integrity of the villi were visible in groups B and C. Edema of the submucosa and circular muscle was observed in all groups. Hemorrhage was observed in different layers for groups B and C, but group C showed more severe hemorrhage in different layers during reperfusion. All groups showed polymorphonuclear leukocyte infiltration on the base of the mucosa, submucosa, and longitudinal muscle, in addition to polymorphonuclear leukocytes margination in the mucosal and submucosal vessels. Necrosis of enterocytes, muscles, crypts of Lieberkühn and myenteric plexus was observed in groups B and C during reperfusion. Topical and intraluminal Carolina Rinse Solution did not attenuate the effects of ischemia and reperfusion in the small intestine of rabbits.

摘要
将15只新西兰成年家兔随机分为三组：假手术组（A组）、缺血再灌注组（B组）及卡罗琳冲洗液（Carolina Rinse Solution, CRS）组（C组）。B组与C组均接受1小时缺血及2小时再灌注造模。C组于再灌注前10分钟，将肠腔充盈卡罗琳冲洗液，并将对应肠段浸泡于该冲洗液中。B组与C组均可见小肠绒毛坏死及结构完整性丧失。所有组别均观察到黏膜下层与环肌水肿。B组与C组各肠壁层均可见出血表现，但C组再灌注期间各层出血程度更为显著。所有组别均可见黏膜基底部、黏膜下层及纵肌存在多形核白细胞浸润，此外黏膜与黏膜下层血管内可见多形核白细胞附壁。再灌注期间，B组与C组可见肠上皮细胞、肌层、利贝库恩隐窝（crypts of Lieberkühn）及肌间神经丛（myenteric plexus）坏死。局部及肠腔内应用卡罗琳冲洗液并未减轻兔小肠缺血再灌注损伤。