<b>Assisted gene flow management to climate change in the annual legume </b><b><i>Lupinus angustifolius </i></b><b>L.: from phenotype to genotype</b>

Our study aimed to evaluate the benefits and limitations of assisted gene flow in enhancing the evolutionary potential of <i>Lupinus angustifolius </i>L. (Fabaceae), considering both phenotypic and genomic perspectives. We collected seeds from four populations in Spain at two latitudes (north and south), and grew them in a common garden. We used pollen from southern individuals to pollinate northern plants and create an F1 gene flow line that would advance its flowering onset. In the next season, we allowed F1 plants to self-pollinate creating an F2 self-pollination line. We also created a backcross line by pollinating control northern plants with pollen from F1 plants. We measured flowering onset, reproductive success, and other plant traits in all resulting lines.本研究旨在从表型与基因组两个视角，评估辅助基因流在提升狭叶羽扇豆（*Lupinus angustifolius* L.，豆科Fabaceae）进化潜力方面的优势与局限。我们从西班牙两个纬度（北部与南部）的4个种群中采集种子，并于同质种植园（common garden）中培育这些植株。我们以南部个体的花粉对北部植株进行授粉，构建F1代基因流株系以提早其开花始期。在下一个生长季，我们让F1代植株自交，构建F2代自交株系。此外，我们以F1代植株的花粉对对照北部植株进行授粉，构建回交株系。我们对所有衍生株系的开花始期、繁殖成功率及其他植株性状进行了测定。