[Data Record 1]: qPCR validations

DESCRIPTION qPCR was used to assess the enrichment of ChIP and MeDIP experiments and to validate the integrity of sequencing libraries FILES Validation of ChIP-seq library enrichment: 20130426-CA1-1h-K27ac-K79me3-K4me3-H3-lib_qPCR.xlsx - validation of ChIP-seq libraries for samples from CA1 taken 1 hour after conditioning and immunoprecipitated for H3K27ac, H3K79me3, H3K4me3 or H3 20130715-ACCnaiveK76me3K27ac_qPCR.xlsx - validation of ChIP-seq libraries for naive samples from ACC and immunoprecipitated for H3K76me3 or H3K27ac 20131125-CA1-Naive_qPCR.xlsx - validation of ChIP-seq libraries for naive samples from CA1 20150122-CA1-K9ac_qPCR.xlsx - validation of ChIP-seq libraries for naive samples from CA1 and immunoprecipitated for H3k9ac Validation of MedIP-seq library enrichment: 20150729-MeDIP-LIB-ACC_qPCR.xlsx - MedIP-seq library validated for genes Cobl, Loxhd1, Vrk1 and GAPDHenh (-) 20150802-ACC-lib_qPCR.xlsx - MedIP-seq library validated for genes Nova1 and Reelin DATA GENERATION qPCR experiments were run on Roche LighCycler 480, and the machine was used to export the Ct values. The resulting output was imported into pyQPCR (version 0.10dev) using dilutions of inputs as standards, along with the following parameters: Calculation type: Absolute quantification Confidence interval: 90.00% Machine: Roche LighCycler 480 Maximum E(Ct): 0.30 Minimum Ct: 35.00

数据集描述：本数据集采用定量聚合酶链反应（quantitative PCR, qPCR）评估染色质免疫共沉淀（Chromatin Immunoprecipitation, ChIP）与甲基化DNA免疫共沉淀（Methylated DNA Immunoprecipitation, MeDIP）实验的富集效果，并验证测序文库的完整性。

相关文件：
### ChIP-seq文库富集验证文件
1. `20130426-CA1-1h-K27ac-K79me3-K4me3-H3-lib_qPCR.xlsx`：用于验证条件刺激1小时后取自CA1脑区样本的ChIP-seq文库，该样本分别针对H3K27ac、H3K79me3、H3K4me3及H3完成免疫沉淀。
2. `20130715-ACCnaiveK76me3K27ac_qPCR.xlsx`：用于验证取自前扣带回皮层（Anterior Cingulate Cortex, ACC）的未处理样本的ChIP-seq文库，该样本分别针对H3K76me3与H3K27ac完成免疫沉淀。
3. `20131125-CA1-Naive_qPCR.xlsx`：用于验证取自CA1脑区未处理样本的ChIP-seq文库。
4. `20150122-CA1-K9ac_qPCR.xlsx`：用于验证取自CA1脑区未处理样本的ChIP-seq文库，该样本针对H3K9ac完成免疫沉淀。

### MeDIP-seq文库富集验证文件
1. `20150729-MeDIP-LIB-ACC_qPCR.xlsx`：针对Cobl、Loxhd1、Vrk1及GAPDHenh（-）基因完成富集验证的前扣带回皮层（ACC）MeDIP-seq文库。
2. `20150802-ACC-lib_qPCR.xlsx`：针对Nova1与Reelin基因完成富集验证的前扣带回皮层（ACC）MeDIP-seq文库。

数据生成流程：qPCR实验在罗氏（Roche）LightCycler 480仪器上开展，由仪器直接导出循环阈值（Cycle Threshold, Ct）值。将所得结果导入pyQPCR（版本0.10dev）进行分析，以输入样本的稀释梯度作为标准品，采用如下分析参数：
- 计算类型：绝对定量
- 置信区间：90.00%
- 所用仪器：罗氏LightCycler 480
- 最大E(Ct)：0.30
- 最小Ct值：35.00