MolMet Figure 6: Higher blood cholesterol and lower fasting blood glucose and insulin in P446L mice on high energy diets.

A-F) Chronic study of P446L mice on HFD: A. Experimental design. B) Body weight, liver weight and epididymis fat pad weight, (n=7,7). C) No difference in free-feeding blood glucose and insulin (21 wk, n=7,7) or glucose tolerance (4-wk, GTT, n=9,9). D) Higher blood cholesterol and liver triglyceride by LL genotype but no difference in blood triglyceride or liver cholesterol (n=6-7). E) Representative hematoxylin-eosin images showing microvesicular steatosis in LL-genotype. F) Histopathology scores for steatosis, microvesicular steatosis, lipogranulomas, lobular Inflammation, portal Inflammation and fibrosis showing higher microvesicular steatosis by LL genotype (n=7,7). G-L) Chronic study of P446L mice on HFHSD: G. Experimental design. H. Body weight, liver weight and higher epididymal fat pad wt by LL genotype, n=11-13. I) Free feeding blood glucose and insulin and glucose tolerance test (GTT), n=11-14. J) Lower post-prandial blood glucose (2h food withdrawal) and insulin and inorganic phosphate (Pi) at cull, n=10-14. K) Higher blood cholesterol in LL genotype but no difference in blood triglycerides or liver triglycerides and cholesterol, n=10-14. L) Histopathology scores showing higher lipogranuloma and lower fibrosis scores by LL genotype, n=14,13. M) Representative hematoxylin-eosin images showing lipogranulomas and Sirius red fast green (SRFG) for fibrosis staining. Categorical data statistical differences was by Chi Square test; other analysis was by t-test #P< 0.05; ##P< 0.01; ### P<0.001 for genotype effect.

A-F) 携带P446L突变的小鼠高脂饮食（High Fat Diet, HFD）慢性研究：A. 实验设计。B. 体质量、肝质量及附睾脂肪垫质量（n=7,7）。C. 自由进食状态下的血糖、胰岛素水平（21周龄，n=7,7）以及葡萄糖耐量试验（Glucose Tolerance Test, GTT，4周龄，n=9,9）均无组间差异。D. LL基因型小鼠的血胆固醇及肝甘油三酯水平更高，但血甘油三酯与肝胆固醇水平无组间差异（n=6-7）。E. 代表性苏木精-伊红（Hematoxylin-Eosin, HE）染色图像显示LL基因型小鼠存在微泡性脂肪变。F. 脂肪变、微泡性脂肪变、脂肪肉芽肿、小叶性炎症、汇管区炎症及纤维化的组织病理学评分结果显示，LL基因型小鼠的微泡性脂肪变程度更高（n=7,7）。
G-L) 携带P446L突变的小鼠高脂高糖饮食（High Fat High Sugar Diet, HFHSD）慢性研究：G. 实验设计。H. 体质量、肝质量，且LL基因型小鼠附睾脂肪垫质量更高（n=11-13）。I. 自由进食状态下的血糖、胰岛素水平及葡萄糖耐量试验（GTT）结果（n=11-14）。J. 处死前禁食2小时状态下的血糖、胰岛素水平及无机磷（Inorganic Phosphate, Pi）水平更低（n=10-14）。K. LL基因型小鼠血胆固醇水平更高，但血甘油三酯、肝甘油三酯及肝胆固醇水平无组间差异（n=10-14）。L. 组织病理学评分结果显示，LL基因型小鼠的脂肪肉芽肿评分更高，而纤维化评分更低（n=14,13）。M. 代表性苏木精-伊红染色图像显示脂肪肉芽肿，以及天狼星红固绿（Sirius Red Fast Green, SRFG）纤维化染色图像。
分类数据的统计学差异采用卡方检验；其余分析采用t检验，#P<0.05、##P<0.01、###P<0.001表示基因型效应存在统计学差异。