Dissecting cell composition and cell-cell interaction network of normal human heart tissue by single-cell sequencing

We studied the cell compositon of normal human heart by single-cell sequencing. Distint subgroups of cardiac muscle, fibroblast cell and endothelial cell were detected. We drawed a cell-cell interaction network using specific expressed ligands and receptors of cells. And we also observed the change of interaction and cell transformation with age. Extract cells from left ateria and ventricle of normal heart and conduct single-cell sequencing of 9994 cells.

本研究通过单细胞测序（single-cell sequencing）技术分析了正常人类心脏的细胞组成，检测到心肌细胞（cardiac muscle cell）、成纤维细胞（fibroblast cell）与内皮细胞（endothelial cell）的不同亚群。我们利用各细胞特异性表达的配体（ligand）与受体（receptor）构建了细胞间相互作用网络，并观察到随年龄增长而发生的细胞相互作用变化与细胞转化现象。本研究从正常心脏的左动脉与左心室中提取细胞，对共计9994个细胞开展了单细胞测序。