Tissue specific expression in Cuscuta campestris haustoria using laser-capture microdissection (LCM)

To characterize Cuscuta haustorium developmental stages, we used laser-capture microdissection (LCM) with RNA-seq to analyze pure haustorial tissues from three different developmental stages. We focused on 4 DPA and defined three developmental stages based on the haustorium structure: early (the haustorium has just contacted the host), intermediate (the haustorium has developed searching hyphae but has not formed vascular connections), and mature (a mature haustorium with continuous vasculature between host and parasite).

为表征菟丝子（Cuscuta）吸器（haustorium）的发育阶段，我们采用激光捕获显微切割（Laser-capture microdissection, LCM）联合RNA测序（RNA-seq）技术，对三个不同发育阶段的纯吸器组织进行分析。本研究以开花后4天（4 DPA）为研究基准，并依据吸器的结构特征划分为三个发育阶段：早期阶段（吸器刚与寄主建立接触）、中间阶段（吸器已形成搜索菌丝但尚未建立维管连接）以及成熟阶段（寄主与寄生植物间具备连续维管系统的成熟吸器）。