Rooting of Tineke and Natal Briar rose stem cuttings in different substrates

ABSTRACT: The commercial propagation of rose bushes is carried out asexually by the cutting method. The genetic material and the substrate are factors that interfere in the rhizogenic process of the cuttings. Thus, the aim of this work was to evaluate the effect of the substrate on the rooting of cuttings of two hybrid rose bushes. Floriferous branches of matrix plants were cut at the apex and at the base, approximately 8cm long and with two buds, and the basal leaf was removed. The experimental design was completely randomized, in a 2 × 3 factorial scheme (two cultivars, Tineke and Natal Briar × three substrates, carbonized rice husk, coconut fiber and TN Gold®) containing four replicates of 15 cuttings per replicate. The base of the cuttings was immersed for five seconds in IBA 2000mg L-1 solution (indole butyric acid). The cuttings were placed in trays with 24 cells previously filled with substrate and deposited on the floor in a transparent plastic tunnel, built inside a stove. After the 50-day experiment, rootstocks (%), sprouts (%) and dead (%), root length (cm), fresh and dry shoot biomass and roots (g) were evaluated. The rooting percentage of the Natal Briar cultivar was 75%, thus superior to the Tineke cultivar, which had a rooting percentage of 39%. The best substrates for the Tineke and Natal Briar rose cuttings are CAC and coconut fiber, which provided rooting percentages of 81% and 67% of the cuttings, respectively.

摘要：玫瑰灌木的商业化繁殖多采用扦插方式进行无性繁殖。遗传物质与基质是影响插穗生根过程的关键因素。本研究旨在探究基质对两个杂交玫瑰灌木品种插穗生根的影响。
采集供试母株的开花枝条，剪取顶端与基部段，长度约8cm，保留2个芽点，并去除基部叶片。试验采用完全随机设计，设置2×3因子方案：2个品种（蒂尼克（Tineke）与纳塔尔布里亚尔（Natal Briar））×3种基质（碳化稻壳、椰糠与TN Gold®），每处理设置4次重复，每次重复含15根插穗。将插穗基部于2000mg·L⁻¹的吲哚丁酸（indole butyric acid，IBA）溶液中浸泡5秒。将插穗移栽至预先装填好基质的24孔托盘内，放置于温室内搭建的透明塑料拱棚地面上。
试验持续50天后，测定插穗的生根率（%）、萌芽率（%）、死亡率（%）、根长（cm）、地上部与根系的鲜重及干重（g）。结果显示，纳塔尔布里亚尔品种的插穗生根率为75%，显著高于生根率仅39%的蒂尼克品种。适配蒂尼克与纳塔尔布里亚尔玫瑰插穗的最优基质分别为碳化稻壳（CAC）与椰糠，二者对应的插穗生根率分别可达81%与67%。