Minimal data set.

Background/Objectives
Obesity is a chronic metabolic disorder, and its prevalence in Bangladesh is increasing at an alarming rate. Previous reports have suggested a significant association between Vitamin D receptor (VDR) gene polymorphisms and obesity, but with inconsistent results. The purpose of our study was to investigate the association between two single-nucleotide polymorphisms (SNPs) (Apal, rs7975232, and Taql, rs731236) of the VDR gene and the risk of obesity in the Bangladeshi population. Moreover, we looked at serum VDR levels and serum 25-hydroxy vitamin D levels in people with obesity (n = 124) and healthy controls (n = 126).
Methods
Genotyping was performed using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP). General linear model and multivariate logistic regression analysis were used to calculate the adjusted odds ratio (OR) along with 95% confidence intervals (CI) and P-values.
Results
Serum VDR level was downregulated in people with obesity compared to healthy controls (P < 0.0001) along with significantly lower levels of 25-hydroxy vitamin D (P < 0.0001). For the ApaI rs7975232 (C > A) polymorphism, the CA Heterozygous genotype carried a 1.93-fold higher risk of developing obesity (OR=1.93, 95% CI = 1.10–3.41, P = 0.023). On the contrary, for TaqI, rs731236 (T > C), no significant association was found for both heterozygous and mutant homozygous genotypes.
Conclusion
We report the downregulation of serum VDR levels and serum 25-hydroxy vitamin D levels in people with obesity. Moreover, a polymorphism of Apal (rs7975232 C > A) in the VDR gene increases the risk of developing obesity in the Bangladeshi population.

背景与研究目标
肥胖是一种慢性代谢紊乱性疾病，其在孟加拉国的患病率正以惊人的速度攀升。既往研究提示维生素D受体（VDR）基因多态性与肥胖存在显著关联，但相关研究结果并不一致。本研究旨在探究孟加拉国人群中VDR基因的两个单核苷酸多态性（SNPs）——ApaI（rs7975232）与TaqI（rs731236）与肥胖发病风险的关联。此外，本研究还检测了肥胖人群（n=124）与健康对照者（n=126）的血清VDR水平及血清25-羟维生素D（25-hydroxy vitamin D）水平。
研究方法
本研究采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）进行基因分型。通过一般线性模型与多因素logistic回归分析，计算校正后的比值比（OR）及其95%置信区间（CI）与P值。
研究结果
与健康对照者相比，肥胖人群的血清VDR水平显著下调（P<0.0001），血清25-羟维生素D水平亦显著降低（P<0.0001）。针对ApaI rs7975232（C>A）多态性，CA杂合基因型个体的肥胖发病风险升高1.93倍（OR=1.93，95%CI=1.10–3.41，P=0.023）。与之相反，针对TaqI rs731236（T>C），无论是杂合基因型还是突变纯合基因型，均未发现与肥胖发病风险存在显著关联。
研究结论
本研究证实，肥胖人群的血清VDR与血清25-羟维生素D水平均存在下调现象。此外，VDR基因中ApaI（rs7975232 C>A）多态性会增加孟加拉国人群的肥胖发病风险。