GNPS - Comprehensive Untargeted Lipidomic Profiling of Third Generation Lentiviral Vectors and Packaging Cells: Raw LCMS Data

Untargeted high-resolution lipidomics data of HEK 293T packaging cells and lentiviral vectors (LV) in cell culture media. HEK 293T cells are treated with either vehicle control (DMSO), myriocin (positive control), expressing the VSV G protein or producing 3rd generation LV. LV are concentrated from HEK 293T cell culture media using ultracentrifugation. HEK 293T culture media from untransfected cells is also concentrated by ultracentrifugation and acquired as a lipidomics blank which is used to subtract the abundance of background lipids from the LV samples. Also included, is a diltution series of fetal bovine serum lipid extract (FBS) which is used to asses the linear dynamic range of the instrument.

本数据集涵盖HEK 293T包装细胞及细胞培养基中慢病毒载体（lentiviral vectors, LV）的非靶向高分辨率脂质组学（lipidomics）数据。实验中，HEK 293T细胞分别经溶剂对照（二甲基亚砜, DMSO）、多球壳菌素（myriocin，阳性对照）处理，或转染以表达水疱性口炎病毒G蛋白（VSV G protein），或用于制备第三代慢病毒载体（3rd generation LV）。慢病毒载体通过超速离心法（ultracentrifugation）从HEK 293T细胞培养基中富集得到。未转染HEK 293T细胞的培养基同样经超速离心富集后，作为脂质组学空白对照用于扣除慢病毒载体样品中的本底脂质丰度。此外，本数据集还包含胎牛血清脂质提取物（fetal bovine serum lipid extract, FBS）的梯度稀释系列，用于评估仪器的线性动态范围。