The type and relative distribution of the PP isolated RNAs by RNA-sequencing analysis in HeLa cells and dynamic investigation upon transcription inhibition by ActD treatment. The type and relative distribution of the PP isolated RNAs by RNA-sequencing analysis in HeLa cells and dynamic investigation upon transcription inhibition by ActD treatment

Developing a psoralen probe (PP)-based method for RNA tagging and RNA-protein complex (RP-complex) enrichment, isolating of both coding and noncoding RNAs from HeLa cells was achieved by PP enrichment. Exploring the type and relative distribution of the PP isolated RNAs by RNA-sequencing analysis. And dynamic investigation of RNA upon transcription inhibition by ActD treatment. Overall design: The type and relative distribution of RNAs in HeLa cells by PP enrichment and TRIzol, RNA readcounts in HeLa cells treating with ActD

本研究开发了一种基于补骨脂素探针（psoralen probe, PP）的RNA标记与RNA-蛋白质复合物（RNA-protein complex, RP-complex）富集方法，通过该PP富集策略可从海拉细胞中分离得到编码RNA与非编码RNA。通过RNA测序（RNA-sequencing）分析，探究PP分离所得RNA的类型与相对分布特征。同时对经放线菌素D（ActD）处理、处于转录抑制状态下的RNA进行动态监测。整体实验设计：分别通过PP富集法与TRIzol法提取海拉细胞中的RNA，分析其RNA类型与相对分布；并检测经ActD处理的海拉细胞的RNA读段计数。