RNA-seq of Arabidopsis thaliana seedlings overexpressing the transcription factor TCP14. Arabidopsis thaliana

This study evaluates the transcriptome of transgenic Arabidopsis seedlings expressing the transcription factor TCP14 (AT3G47620) under the UBQ10 promoter. Overall design: Arabidopsis thaliana seedlings were grown under short day conditions (9h of light at 21°C - 15h of dark at 18°C) for two weeks. Samples were harvested in the beginning of the light period and used for RNA extraction and Illumina sequencing. Lines Col-0 (4 replicates), UBQ10::YFP-TCP14-3 (4 replicates), UBQ10::YFP-TCP14-4 (1 replicate), and coi1-16 (2 replicates) were used in the experiment. Each replicate corresponds to approximately 30 seedlings grown in the same pot.

本研究对在UBQ10启动子（UBQ10 promoter）下表达转录因子TCP14（AT3G47620）的转基因拟南芥幼苗的转录组（transcriptome）进行了分析评估。实验设计概要：将拟南芥（Arabidopsis thaliana）幼苗置于短日照培养条件（21℃下光照9小时、18℃下黑暗15小时）中培养两周。于光照周期起始时刻采集样本，用于RNA提取及Illumina测序。本实验使用的材料包括Col-0（4次生物学重复）、UBQ10::YFP-TCP14-3（4次生物学重复）、UBQ10::YFP-TCP14-4（1次生物学重复）以及coi1-16（2次生物学重复）。每个生物学重复对应约30株生长于同一培养盆的幼苗。