Characterization of human ILC2 subsets. Characterization of human ILC2 subsets

To characterize a novel human ILC2 subset found in peripheral blood, RNAseq was performed. Other ILC subsets from blood and tonsil were also included. Overall design: ILC subsets were sort purified, and immediately lysed for RNA extraction. RNAseq was performed using SmartSeq technology.为表征在外周血中发现的新型人类2型固有淋巴细胞（Innate Lymphoid Cell 2, ILC2）亚群，本研究开展了RNA测序（RNA-seq）实验。此外，本研究还纳入了取自血液与扁桃体的其他固有淋巴细胞（Innate Lymphoid Cell, ILC）亚群。整体实验设计如下：对各ILC亚群进行分选纯化后，立即裂解以提取RNA，并采用SmartSeq技术完成RNA测序。