Data for: Comparative proteomics of geographically distinct saw-scaled viper (Echis carinatus) venoms from India

We investigated on venom variation in geographically distinct populations of Echis carinatus from three ecologically distinct regions: Tamil Nadu (ECVTN), Goa (ECVGO), and Rajasthan (ECVRAJ). Venom was fractionated by RP-HPLC, combined with SDS-PAGE, and subjected to tandem mass spectrometry. Toxins were identified, and their relative abundance was estimated. Using NCBI database of Echis genus, we queried the MS/MS spectra, and found 87, 44 and 48 proteins, in ECVTN, ECVGO and ECVRAJ respectively, belonging to 7-9 different toxin families. The differences in the venom profiles were due to change in the relative composition of the toxin families.

本研究针对来自三个生态迥异区域的地理分隔锯鳞蝰（Echis carinatus）种群的毒液变异情况展开探究，三个区域分别为泰米尔纳德邦（ECVTN）、果阿邦（ECVGO）与拉贾斯坦邦（ECVRAJ）。研究采用反相高效液相色谱（RP-HPLC）结合十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）对毒液进行分级分离，并通过串联质谱（tandem mass spectrometry）进行分析；完成毒素鉴定的同时，对其相对丰度进行了定量估算。本研究依托锯鳞蝰属（Echis genus）的美国国家生物技术信息中心（NCBI）数据库，对串联质谱（MS/MS）谱图进行检索，结果显示ECVTN、ECVGO及ECVRAJ样本中分别鉴定出87、44和48种蛋白质，这些蛋白质隶属于7至9个不同的毒素家族。毒液谱型的差异源于各毒素家族相对组成的变化。