snRNA-seq of Hexb-deficient brain cells upon microglia replacement

We used snRNA-sequencing to analzye NeuN-Olig2- thalamic brain cells derived from Hexb-deficient mice upon microglia replacement Overall design: CNS nuclei were isolated from frozen thalamic specimen, FACS purified, and analyzed with snRNA-seq

本研究采用单细胞核RNA测序（snRNA-sequencing），分析经小胶质细胞替换处理后的Hexb基因缺陷型小鼠来源的NeuN阴性、Olig2阴性丘脑脑细胞。实验整体设计：从冻存的丘脑标本中分离中枢神经系统（Central Nervous System, CNS）细胞核，经荧光激活细胞分选（Fluorescence Activated Cell Sorting, FACS）纯化后，采用单细胞核RNA测序完成检测分析。