Genome-wide chromatin binding of YBX1 in nasopharyngeal carcinoma (NPC) cell line S18 (ChIP-Seq)

The development of therapeutic resistance and metastatic dissemination takes place in 20-30% patients with nasopharyngeal carcinoma (NPC), resulting in poor survival. Hence, a better understanding of the underlying molecular mechanisms will help improve clinical outcome. We have identified a novel circRNA, circIPO7, as a promoter of metastasis and cisplatin chemoresistance in NPC cells. circIPO7 was found to bind to YBX1. Genome binding/occupancy profiling by high throughput sequencing were performed to explore the underlying molecular mechanisms. Overall design: Chromatin Immunoprecipitation (ChIP) was performed in the human nasopharyngeal carcinoma cell line S18 using YBX1 antibody, and then subjected to ChIP sequencing.

鼻咽癌（nasopharyngeal carcinoma, NPC）患者中有20%~30%会出现治疗耐药与转移播散，进而导致不良生存结局。因此，深入解析其潜在分子机制有助于改善临床治疗效果。本研究已鉴定出一种新型环状RNA（circRNA）circIPO7，其可促进鼻咽癌细胞的转移与顺铂化疗耐药。研究发现circIPO7能够与YBX1结合。为探究其潜在分子机制，我们通过高通量测序开展了基因组结合与占据谱分析。整体实验设计：使用YBX1抗体对人鼻咽癌细胞系S18进行染色质免疫共沉淀（Chromatin Immunoprecipitation, ChIP），随后进行ChIP测序。