The transcriptomic landscape of spinal V1 interneurons reveals a role for En1 in specific elements of motor output

We present a transcriptomic atlas of spinal V1 interneurons across postnatal development (P0, P14, P28, and P56) in the mouse using single-nucleus RNA sequencing (snRNA-seq). We also present transcriptomic data comparing V1 interneurons in En1 heterozygous and En1 KO mice at age P0 only. V1 nuclei were genetically labeled by crossing En1::Cre mice in either the heterozygous or homozygous (KO) state to RC.lsl.Sun1-sfGFP (INTACT) mice, and GFP+ nuclei were isolated by fluorescence activated cell sorting. snRNA-seq was performed on the 10X Genomics Chromium 3' platform (v3.1).