The transcriptomic landscape of spinal V1 interneurons reveals a role for En1 in specific elements of motor output

We present a transcriptomic atlas of spinal V1 interneurons across postnatal development (P0, P14, P28, and P56) in the mouse using single-nucleus RNA sequencing (snRNA-seq). We also present transcriptomic data comparing V1 interneurons in En1 heterozygous and En1 KO mice at age P0 only. V1 nuclei were genetically labeled by crossing En1::Cre mice in either the heterozygous or homozygous (KO) state to RC.lsl.Sun1-sfGFP (INTACT) mice, and GFP+ nuclei were isolated by fluorescence activated cell sorting. snRNA-seq was performed on the 10X Genomics Chromium 3' platform (v3.1).本研究借助单细胞核RNA测序（single-nucleus RNA sequencing, snRNA-seq）技术，构建了小鼠出生后各发育阶段（P0、P14、P28及P56）脊髓V1中间神经元的转录组图谱。此外，本研究还提供了仅针对P0月龄小鼠的En1杂合子与En1敲除（knockout, KO）小鼠脊髓V1中间神经元的转录组对比数据。实验过程中，研究人员将处于杂合或纯合（敲除）状态的En1::Cre小鼠与RC.lsl.Sun1-sfGFP（INTACT）小鼠杂交，实现V1神经元细胞核的遗传标记；随后通过荧光激活细胞分选（fluorescence activated cell sorting, FACS）分离得到GFP阳性细胞核。本研究的单细胞核RNA测序实验均在10X Genomics Chromium 3'测序平台（v3.1版本）上完成。