Microelectrode array voltage stimulation of cocultured fungal ELMs

Voltage-based stimulation of fungal ELM in co-cultures of Hericium erinaceus with Bacillus subtilis using custom fabricated microelectrode array with electrode radius of 25 μm and spacing of 700 μm. A pair of dual channel voltage-based stimulation microelectrodes were utilised, located at four outer corners of the array. The analysis examined 48 distinct stimulation conditions at a sample rate of 30 kHz across 48 experimental groups, including 24 biphasic and 24 monophasic stimulation protocols with both cathodic and anodic pulses. Data was collected from multiple trials per condition and stimulus artifact removal was implemented via blanking techniques. This upload includes the raw data for a single condition: biphasic cathodic stimulation for co-cultures of H. erinaceus and B. subtilis. Note that stimulus blanking and other signal processing methods have not been applied to this uploaded raw data file. Recommended data loading is via OpenEphys/spikeinterface Python loaders or similar.采用定制化制备的电极半径为25 μm、电极间距为700 μm的微电极阵列，对猴头菇（Hericium erinaceus）与枯草芽孢杆菌（Bacillus subtilis）共培养体系中的真菌ELM开展电压刺激。本实验采用一对双通道电压刺激微电极，该电极设置于微电极阵列的四个外拐角位置。本次分析覆盖48个实验组，共包含48种独立刺激条件，采样率设定为30 kHz；其中涵盖24种双相刺激方案与24种单相刺激方案，两类方案均包含阴极脉冲与阳极脉冲刺激。每种刺激条件均通过多次重复实验采集数据，并采用消隐技术完成刺激伪迹去除。 本次上传的数据对应单种刺激条件：猴头菇（H. erinaceus）与枯草芽孢杆菌（B. subtilis）共培养体系的双相阴极刺激实验原始数据。请注意，本次上传的原始数据文件未施加刺激消隐及其他信号处理操作。推荐使用OpenEphys/spikeinterface Python加载工具或同类工具进行数据读取。