Microbial communities in different particle sizes

In this study, the collected mangrove sediments were sifted into different groups according to particle size. DNA was extracted from the samples of each group, and 16SrRNA sequencing was performed to obtain the OTU detection results, and community composition was analyzed. After annotation, the microflora at phylum and genus level were mainly observed to observe their distribution composition and changes in different particle sizes. Network analysis was performed to analyze the effects of particle size on species and to show patterns of co-occurrence between species. The diversity results were analyzed to see if there were diversity differences between the Alpha diversity observation groups, and the Beta diversity observation whether different groups were significantly separated. LEfSe analysis observed species with significant differences between groups, combined with element cycling species and genes, to characterize the influence of particle size on the element cycling process. The extracted DNA was sequenced by metagenomic sequencing to study the population structure of microorganisms in different particle sizes, functional activity of genes with functional element cycling, and the relationship between microorganisms and the environment.

本研究将采集所得的红树林沉积物按粒径划分为不同组别。对各组样本提取脱氧核糖核酸（DNA），通过16S核糖体RNA（16SrRNA）测序获得操作分类单元（OTU）检测结果，并开展群落组成分析。经注释后，重点解析门类与属水平的微生物群落，以探究其在不同粒径下的分布组成及变化规律。通过网络分析，探讨粒径对微生物物种的影响，并揭示物种间的共现模式。对多样性结果进行分析：通过α（Alpha）多样性检验各组间的多样性差异，通过β（Beta）多样性判断不同组别是否存在显著分离。采用线性判别分析效应大小（LEfSe）筛选组间具有显著差异的物种，结合元素循环相关物种与基因，表征粒径对元素循环过程的影响。对提取的DNA进行宏基因组测序，以研究不同粒径下微生物的种群结构、参与元素循环的基因功能活性，以及微生物与环境之间的相互关系。