Supplementary dataset for 16S rRNA amplicon sequencing of Oppiella nova (PRJNA1395509)

Supplementary Dataset: 16S rRNA Amplicon Sequencing of Oppiella novaNCBI BioProject: PRJNA1395509 1. FILE DESCRIPTIONSupplementary_Data_Matrix.xlsx — Complete supplementary dataset with 5 sheets Sheet 1: Sample_Metadata - Complete information for all 18 samples - Columns: Sample_ID, Full_Sample_Name, SRA_Run_Accession, BioProject_Accession, Wolbachia_Infection_Status, Developmental_Stage, Biological_Replicate, Host_Species, Sequencing_Platform, Marker_Gene - All raw sequencing reads are publicly archived in NCBI SRA under BioProject PRJNA1395509 Sheet 2: OTU_Abundance_Matrix - Raw OTU abundance matrix (685 OTUs × 18 samples) - Values: raw sequencing read counts - Rows: OTUs; Columns: samples Sheet 3: Taxonomy_Annotation - Full taxonomic classification for all 685 OTUs - Levels: domain, kingdom, phylum, class, order, family, genus, species Sheet 4: Phylum_Level_Abundance - Aggregated abundance table at phylum level (22 phyla × 18 samples) - Values: summed raw read counts per phylum Sheet 5: Genus_Level_Abundance - Aggregated abundance table at genus level (376 genera × 18 samples) - Values: summed raw read counts per genus 2. EXPERIMENTAL DESIGN- Host species: Oppiella nova (soil mite)- Experimental factors: * Wolbachia infection status: Infected / Uninfected * Developmental stage: Larva / Nymph / Adult- Biological replicates: 3 per group- Total samples: 18 (6 groups × 3 replicates)- Sequencing: 16S rRNA gene amplicon, Illumina MiSeq Sample groups: - P1-P3: Larva, Wolbachia-infected (3 replicates) - V1-V3: Larva, Wolbachia-uninfected (3 replicates) - L1-L3: Nymph, Wolbachia-infected (3 replicates) - H1-H3: Nymph, Wolbachia-uninfected (3 replicates) - J1-J3: Adult, Wolbachia-infected (3 replicates) - T1-T3: Adult, Wolbachia-uninfected (3 replicates) 3. DATA AVAILABILITY- Raw sequencing reads (fastq files): NCBI SRA, BioProject PRJNA1395509 18 SRA runs: SRX31650419 – SRX31650436- Processed analytical data: This repository 4. SRA ACCESSION LIST P1: SRX31650430 P2: SRX31650431 P3: SRX31650432 V1: SRX31650419 V2: SRX31650420 V3: SRX31650429 L1: SRX31650436 L2: SRX31650421 L3: SRX31650422 H1: SRX31650433 H2: SRX31650434 H3: SRX31650435 J1: SRX31650426 J2: SRX31650427 J3: SRX31650428 T1: SRX31650423 T2: SRX31650424 T3: SRX31650425