The effect of prohibitin overexpression in Caco2-BBE intestinal epithelial cells

To determine the effect of prohibitin overexpression on global gene expression in Caco2-BBE intestinal epithelial cells. 4 individual wells of Caco2-BBE cells, passage 41, were transfected with either empty vector (pcDNA4) or prohibitin/pcDNA4 for 72 hours. Total RNA isolated from 4 wells of cells/per treatment were pooled together for labeling and hybridization purposes.

为探究增殖抑制素（prohibitin）过表达对Caco2-BBE肠上皮细胞全局基因表达的影响，实验选取4个独立培养孔的第41代Caco2-BBE细胞，分别转染空载体（pcDNA4）或增殖抑制素/pcDNA4重组载体，转染处理时长为72小时。随后将每个处理组的4个细胞孔中提取的总RNA混合，用于后续的标记与杂交实验。