Gene expression profiling (total RNA-Seq) of HCV susceptibility Cells, Huh7.5 cell lines and primary human hepatocyte cells

To elucidate the underlying causes associated with HCV infection and proliferation, total RNA sequencing was conducted to compare transcriptome levels in primary human hepatocytes (PHH) and Huh7.5 cells, which differ in HCV susceptibility. Corning® Gentest™ Plateable Human CryoHepatocytes (Cat. no 454541, Lot no 393B) were utilized for primary human hepatocytes (PHH), and the Huh7.5 cell line was used for the human hepatoma cells. Total RNA was isolated from both cell types using Trizol reagent (Invitrogen), followed by total RNA sequencing.

为阐明与丙型肝炎病毒（HCV）感染及增殖相关的潜在机制，本研究通过总RNA测序，对比了对HCV易感性存在差异的原代人肝细胞（PHH）与Huh7.5细胞的转录组水平。原代人肝细胞（PHH）采用康宁（Corning®）Gentest™ 贴壁型人冷冻肝细胞（货号：454541，批号：393B），人肝癌细胞则使用Huh7.5细胞系。研究人员采用Trizol试剂（Invitrogen）从两类细胞中提取总RNA，随后开展总RNA测序。