Gene expression analysis in AML systems after 6 hours of HDACi treatments

K562, U937 and NB4 AML cell lines were treated with 2 HDACi, MS-275 and SAHA, and gene expression profiles were analysed to 6-hours treatments. The analysis revealed commonly regulated genes in the 3 cell lines and by 2 HDACi. Total RNA were obtained from 3 AML cell lines after 6 hours of treatment with SAHA and MS-275 and gene expression profiles were compared to untreated cells (control).

本数据集针对K562、U937及NB4三种急性髓系白血病（AML, Acute Myeloid Leukemia）细胞系，采用MS-275与SAHA两种组蛋白去乙酰化酶抑制剂（HDACi, Histone Deacetylase Inhibitors）进行6小时处理，并对其基因表达谱展开分析。具体实验中，研究人员从经SAHA及MS-275处理6小时的三种AML细胞系中提取总RNA，将所得基因表达谱与未处理的对照组细胞进行比对，最终鉴定出在三种细胞系中均受两种HDACi共同调控的基因。