(A) Biological replicates of fluorescent immunoblots performed in control conditions (Ct) are shown (Replicates 1 and 2). The portions of the images used in the main text are outlined. (B) Biological
IGF peptides (recombinant mouse IGF-II or synthetic mouse vesiculin) were biotinylated following manufacturers’ instructions. Either 40ng or 20ng mouse IGFBP of each IGFBP (1, 2, 3, 5 and 6) was dotte
This dataset includes uncropped blots for all experimental replicates that are represented in Figure 1. Treatments and immunoblot methods were performed as outlined in Figure 1. Blots were probed with
