SH1403591.10FU

UNITE provides a unified way for delimiting, identifying, communicating, and working with DNA-based Species Hypotheses (SH). All fungal ITS sequences in the international nucleotide sequence databases are clustered to approximately the species level by applying a set of dynamic distance values (<0.5 - 3.0%). All species hypotheses are given a unique, stable name in the form of a DOI, and their taxonomic and ecological annotations are verified through distributed, web-based third-party annotation efforts. SHs are connected to a taxon name and its classification as far as possible (phylum, class, order, etc.) by taking into account identifications for all sequences in the SH. An automatically or manually designated sequence is chosen to represent each such SH. These sequences are released (https://unite.ut.ee/repository.php) for use by the scientific community in, for example, local sequence similarity searches and next-generation sequencing analysis pipelines. The system and the data are updated automatically as the number of public fungal ITS sequences grows.

UNITE提供了一套统一的方法，用于界定、识别、传播及处理基于DNA的物种假说（DNA-based Species Hypotheses）。国际核苷酸序列数据库中的所有真菌内转录间隔区（Internal Transcribed Spacer，ITS）序列，通过应用一组动态距离阈值（0.5%至3.0%），被聚类至近似物种级别的单元。所有物种假说均会被赋予唯一且稳定的数字对象标识符（Digital Object Identifier，DOI）作为名称，其分类学与生态学注释将通过分布式的网络化第三方注释流程完成核验。此类物种假说将尽可能关联至对应的分类单元名称及其分类层级（如门、纲、目等），关联过程需综合考量该物种假说下所有序列的鉴定结果。随后将选取一条自动或人工指定的序列，作为每个物种假说的代表序列。这些代表序列已发布于https://unite.ut.ee/repository.php，可供科研社群用于本地序列相似性搜索、新一代测序分析流程等科研场景。随着公共真菌ITS序列数量的增长，该系统与配套数据将自动进行更新。