Analysis of wheat straw grown Trichoderma reesei gene expression as influenced by L-methionine in the medium

Wheat straw grown cultures of T. reesei QM9414 were supplemented with 100 µM L-methionine and the genome wide gene expression monitored in order to find novel L-Methionine repressible genes. Total RNA was isolated from independent duplicate shake flask cultures of T. reesei QM9414 pregrown on pretreated wheat straw. Global gene and analyzed using a 4 chip design where 2 chips each represented cultures with or without exogeneously added 100 µM L- Methionine.

以小麦秸秆为培养基质培养的里氏木霉（T. reesei）QM9414菌株培养液中添加100 µM L-甲硫氨酸（L-methionine），并对其全基因组基因表达水平进行监测，以筛选新型的L-甲硫氨酸抑制型基因。从经预处理小麦秸秆预培养的独立重复摇瓶培养物中提取总RNA，采用4芯片设计开展全基因组基因表达分析，其中每2张芯片分别对应添加与未添加100 µM外源L-甲硫氨酸的培养组。