Jakyak-gamcho-tang ameliorates dexamethasone-induced muscle atrophy and muscle dysfunction [C2C12]. Jakyak-gamcho-tang ameliorates dexamethasone-induced muscle atrophy and muscle dysfunction [C2C12]

Jakyak-gamcho-tang (JGT) is a herbal medicine that has been traditionally prescrived for pain control including muscular pain. This transcriptome analysis aims to investigate the changes in global gene expression profile in differentiated murine C2C12 myotube cells treated with JGT extract. Overall design: Comparative gene expression profiling analysis of RNA-seq data for C2C12 myotubes treated with increasing doses of JGT (20, 100, and 500 μg/mL) or vehicle control (0.1% DMSO) for 24 hrs. The dried powders of Paeoniae radix and Glycyrrhizae radix et rhizoma at 1:1 weight ratio was subjected to extraction in a 10-fold volume of hot distilled water for 3 h in a reflux extraction system. The extract solution was filtered through a 53 micrometer mesh filter, concentrated in a rotary evaporator system and lyophilized in a freeze dryer to get a final JGT extract. The JGT stock solution was prepared at 10 mg/mL in PBS containing 2% DMSO.

Jakyak-gamcho-tang（JGT）是一种传统用于缓解包括肌肉疼痛在内的各类疼痛的草药方剂。本转录组分析（transcriptome analysis）旨在探究经JGT提取物处理后的分化小鼠C2C12肌管细胞的全局基因表达谱变化。整体实验设计：对经递增浓度JGT（20、100、500 μg/mL）或溶剂对照（0.1%二甲基亚砜（DMSO））处理24小时的C2C12肌管细胞的RNA测序（RNA-seq）数据开展比较基因表达谱分析。提取物制备流程：按1:1重量比称取干燥的赤芍（Paeoniae radix）与甘草（Glycyrrhizae radix et rhizoma）粉末，以10倍体积的热蒸馏水为溶剂，采用回流提取系统提取3小时。所得提取液经53微米孔径滤膜过滤后，通过旋转蒸发仪浓缩，再经冷冻干燥机冻干，最终获得JGT提取物。JGT储备液制备：将JGT提取物以10 mg/mL的浓度溶于含2% DMSO的磷酸盐缓冲液（PBS）中，即得JGT储备液。