Cortex-wide characterization of decision-making neural dynamics during spatial navigation

To assess the spatial and temporal cortical dynamics during freely-moving decision making, we recorded widefield Ca2+ neuronal activity in mice navigating an 8-maze task with two paradigms in the Main cohort. The first required an alternating figure-8 pattern to receive a reward. The second involved a rule change that only rewarded left turns. For each mouse in this Main cohort, the dataset includes the brain imaging and behavioral data as the mice performed the tasks. A cortical state analysis that identified common global patterns of cortex-wide activity was used, and the cortical state data, and now cortical states relate to the behavior, are included. Also included is an analysis of sequences of cortical states during the behavior. There are similar sets of data for a second Naive cohort of mice that were not trained in the tasks. A third cohort of animals, Alt No Tone, provides the cortical imaging and behavioral data in a version of the experiment in which mice were not given a to..., Methods All experiments were approved by the Institutional Animal Care and Use Committee (IACUC) at the University of Minnesota. Mice were housed in a 12hr light-dark cycle, with times of darkness (the period of mouse wakefulness) occurring during the day when experiments were performed. Animal rooms were kept at an ambient temperature of 68-74 °F and 30-70 % humidity. Based on these previous modified T-maze designs, we constructed an automated 8-maze that was able to detect mouse position and deliver a drop of 5 % sucrose solution on either side of the maze after a desired rule was followed. The 8-maze was an 80 x 54 cm rectangular arena constructed using clear acrylic and custom 3D-printed connection pieces (see Figure 1e). The walls of the arena were 12 cm tall, and corridors were 8 cm wide. The internal walls and floor of the maze were covered with a white, textured vinyl sticker to create traction for the mouse and promote higher visual contrast for overhead cameras. Intersections..., **Cortex-wide characterization of decision-making neural dynamics during spatial navigation** Dataset DOI: [10.5061/dryad.mkkwh71f8](https://doi.org/10.5061/dryad.mkkwh71f8) **Description of the data and file structure** This dataset involves wide-field calcium imaging of neuronal activity in the dorsal cerebral cortex of mice as they freely navigate an 8-maze. The cortical neuronal imaging used GCaMP7f. The main experiment is based on two paradigms in the 8-maze task, the first paradigm required the mice to make alternating left and right turns, and the second paradigm required the mice to make left-only turns. The data associated with the alternating paradigm are labeled with 'Alt', and Left-only paradigm with 'LO'. Supplementary control datasets are also provided. These data can be used to identify and characterize features of cortical neuronal activity and processing in relation to mouse behavior in the 8-maze. Across all data sets, 25 mice are included, and various additional fo..., ,为解析自由移动决策过程中皮层的时空动态变化，我们在主队列（Main cohort）小鼠执行含两种实验范式的8字形迷宫任务时，记录了其宽场钙离子（Ca²+）神经元活动。第一种范式要求小鼠遵循交替8字形路径以获取奖励；第二种范式则变更规则，仅对左转行为给予奖励。本主队列的每只小鼠的数据集均包含其执行任务时的脑成像与行为学数据。本研究采用了可识别全皮层活动共同全局模式的皮层状态分析方法，相关皮层状态数据（皮层状态与行为存在关联）亦包含于数据集内。此外，数据集还包含行为过程中皮层状态序列的分析结果。另有针对第二组未接受过任务训练的未训练队列（Naive cohort）小鼠的相似数据集。第三组动物队列Alt No Tone则提供了某一版本实验中的皮层成像与行为学数据，该版本实验中未向小鼠给予…… **实验方法** 所有实验均经明尼苏达大学机构动物护理与使用委员会（IACUC）批准。小鼠饲养于12小时明暗循环环境中，实验开展于白天的黑暗时段（即小鼠的活跃觉醒期）。动物饲养室的环境温度维持在68~74华氏度，湿度为30%~70%。 本研究基于此前改良的T迷宫设计，搭建了自动化8字形迷宫系统，可实时检测小鼠位置，并在小鼠达成指定规则后，在迷宫两侧滴加一滴5%蔗糖溶液作为奖励。该8字形迷宫为80×54 cm的矩形场地，采用透明亚克力板材与定制3D打印连接件搭建（详见图1e）。迷宫围墙高12 cm，通道宽8 cm。迷宫内壁与底面贴有白色纹理乙烯基贴纸，既可为小鼠提供抓地力，也能提升顶置摄像机的视觉对比度。路口…… **空间导航过程中决策相关神经动态的全皮层表征** 数据集DOI：[10.5061/dryad.mkkwh71f8](https://doi.org/10.5061/dryad.mkkwh71f8) **数据与文件结构说明** 本数据集包含小鼠自由穿梭8字形迷宫时，其背侧大脑皮层神经元活动的宽场钙成像数据。本次皮层神经元成像采用GCaMP7f探针。主实验基于8迷宫任务的两种范式：第一种范式要求小鼠交替进行左转与右转动作，第二种范式仅要求小鼠执行左转动作。与交替范式相关的数据标注为'Alt'，仅左转范式的数据标注为'LO'。本数据集同时提供补充对照数据集。上述数据可用于识别并解析小鼠在8迷宫任务中，皮层神经元活动与行为相关的特征及加工过程。本数据集共包含25只小鼠的相关数据，另有更多补充内容……