Acetyl-CoA is a Key Molecule for Nephron Progenitor Cell Pool Maintenance

To uncover the effectors of metabolism-driven NPC differentiation, we compared the proteomic and transcriptomic signatures of renewing E13.5 NPC induced to differentiate with either glycolysis inhibition or Wnt activation. Acetate emerged as a key metabolite that play a role for NPC fate decision during kidney development. Overall design: Comparative gene expression profiling analysis of RNA-seq data and proteomics signatures of isolated NPCs treated with the Wnt agonist CHIR or with YN1-mediated glycolysis inhibition. Freshly isolated E13.5 NPC (Six2+, Cited1+) were cultured for 24h in the presence or absence of either CHIR or YN1 separately. Then, NPCs were harvested and processed for bulk RNA seq or proteomics.为揭示代谢驱动的肾单位祖细胞（Nephron Progenitor Cells, NPC）分化的效应因子，本研究对比了经糖酵解抑制或Wnt激活诱导分化的增殖态E13.5期NPC的蛋白质组与转录组特征。乙酸盐被确定为在肾脏发育过程中调控NPC命运决定的关键代谢物。 整体实验设计：对经Wnt激动剂CHIR处理或经YN1介导的糖酵解抑制处理的分离NPC，开展RNA测序数据与蛋白质组特征的比较基因表达谱分析。将新鲜分离的E13.5期Six2+、Cited1+ NPC分别在添加或不添加CHIR或YN1的培养基中培养24小时，随后收集NPC并进行批量RNA测序或蛋白质组学检测。