Potential mechanisms of zinc sulfate in inhibiting porcine reproductive and respiratory syndrome virus replication: attenuation of oxidative stress, inflammatory response, and apoptosis

Zinc, the second most abundant essential trace element in living organisms, can effectively inhibit RNA viral infections. Porcine reproductive and respiratory syndrome virus (PRRSV) is a single-stranded RNA virus. It is still unknown whether zinc can suppress PRRSV replication and what its mechanism of action is. Overall design: In this study, PRRSV-infected Marc-145 cell model was employed to systematically investigate zinc sulfate's anti-PRRSV mechanisms through integrated multi-omics approaches, including transcriptomics, real-time quantitative PCR, immunoblot, and flow cytometry.

锌是活体内含量第二丰富的必需微量元素，可有效抑制RNA病毒感染。猪繁殖与呼吸综合征病毒（Porcine reproductive and respiratory syndrome virus, PRRSV）为单链RNA病毒。目前尚不明确锌能否抑制PRRSV复制，及其具体作用机制。实验设计：本研究采用PRRSV感染的Marc-145细胞模型，通过整合转录组学（transcriptomics）、实时定量聚合酶链反应（real-time quantitative PCR）、免疫印迹（immunoblot）及流式细胞术（flow cytometry）等多组学手段，系统探究硫酸锌抗PRRSV的作用机制。