Heat shock-induced ribosomal intergenic spacer RNA. Heat shock-induced ribosomal intergenic spacer RNA

Cells adapt to environmental stressors such as heat shock and extracellular acidosis through formation of nuclear membrane-less compartments called Amyloid bodies (A-bodies). Stressors activate formation of Amyloid bodies (A-bodies) via induction of ribosomal intergenic spacer RNA (rIGSRNA). RNA-seq on non-ribosome depleted RNA from human MCF7 cells exposed to heat shock (43C, 30 minutes) revealed the heat shock-specific expression profile of rIGSRNA. Overall design: Expression profile of the ribosomal intergenic spacer (i.e. rIGSRNA) in cells exposed to heat shock

细胞可通过形成名为淀粉样小体（Amyloid bodies, A-bodies）的无核膜细胞核区室，应对热休克、细胞外酸中毒等环境应激源。应激源可通过诱导核糖体基因间间隔区RNA（ribosomal intergenic spacer RNA, rIGSRNA）的表达，激活淀粉样小体的形成。对经热休克（43℃、30分钟）处理的人MCF7细胞的非核糖体耗竭RNA进行RNA测序，揭示了rIGSRNA的热休克特异性表达谱。整体实验设计：分析接受热休克处理的细胞中核糖体基因间间隔区（即rIGSRNA）的表达谱。