Mus musculus isolate:FH1 | breed:ApoE Transcriptome or Gene expression

Depression is an independent risk factor of AS, increasing the risk of death and disability of AS, but the molecular mechanism is not clear, and there is no effective treatment for AS co-depression. GXII is a famous traditional Chinese medicine formula, which is widely used in the clinical treatment of coronary heart disease and can effectively alleviate symptoms such as chest pain and chest tightness in patients. HPLC-MS/MS was used to analyze the main components of GXII in the blood, hippocampus and prefrontal cortex, thus F is confirmed to be the main active component of GXII, which acts as an anti-AS co-depression. Further, the different expression genes (DEGs) in prefrontal cortex and hippocampus of mice treated with GXII and F were analyzed by RNA-seq technique. The results suggest that the anti-AS co-depression mechanism of GXII and F are related to inflammatory pathways.. This study suggests that the anti-inflammatory effect of F is the main mechanism of GXII in the treatment of AS co-depression.

抑郁症是动脉粥样硬化（Atherosclerosis, AS）的独立危险因素，可升高动脉粥样硬化患者的死亡与残疾风险，但目前其分子机制尚未明确，且针对AS共抑郁症尚无有效治疗方案。GXII为经典中药复方，目前已广泛应用于冠心病的临床治疗，可有效缓解患者胸痛、胸闷等临床症状。采用高效液相色谱-串联质谱（High Performance Liquid Chromatography-Tandem Mass Spectrometry, HPLC-MS/MS）技术，对GXII在小鼠血液、海马体及前额叶皮层中的主要成分进行检测分析，进而确认F为GXII发挥抗AS共抑郁症作用的核心活性成分。进一步通过RNA测序（RNA Sequencing, RNA-seq）技术，分析了经GXII与F干预的小鼠前额叶皮层及海马体组织中的差异表达基因（Differentially Expressed Genes, DEGs）。研究结果表明，GXII与F发挥抗AS共抑郁症作用的机制与炎症通路密切相关。本研究证实，F的抗炎效应是GXII治疗AS共抑郁症的核心作用机制。