Mouse oral tumors induced by activation of Ras and p53 mutations. Mus musculus

The p53 gain of function p53R172H promotes accelerated tumor growth and progression to carcinoma. To identify gene expression changes associated with the oncogenic function of mutant p53 we compared the expression profiles of oral tumors induced by activation of oncogenic K-ras and p53 gain- or loss-of-function mutations Oral tumors were induced by activation of endogenous oncogenic K-rasG12D and p53 loss- or gain-of-function mutations (p53R172H) Overall design: Oral tumors from mice carrying the p53R172H mutation or deletion of p53 were collected for gene expression analysis

p53功能获得性（gain of function）突变体p53R172H可加速肿瘤生长并促进其进展为癌。为鉴定与突变型p53致癌功能相关的基因表达变化，本研究对比了由致癌性K-ras激活联合p53功能获得性或功能丧失性（loss of function）突变诱导的口腔肿瘤的表达谱。本研究通过激活内源性致癌突变K-rasG12D，并联合p53功能丧失性或功能获得性（p53R172H）突变诱导口腔肿瘤。实验设计：收集携带p53R172H突变或p53基因敲除的小鼠口腔肿瘤样本，用于基因表达分析。