Genome wide DRIP-seq in E2 stimulated MCF7 cells

We mapped the location of RNA-DNA hybrids in hormone-starved MCF7 breast cancer cells treated with the hormone estradiol (E2.) E2 is a potent transcriptional activator, and causes proliferation in hormone starved cells. We find a significant induction of hybrids at genes known to be regulated by the estrogen receptor. Examination of RNA:DNA hybrid levels in MCF7 cells treated with two different time points of estradiol, and a mock control

本研究对经雌二醇（estradiol，E2）处理的激素饥饿型MCF7乳腺癌细胞内的RNA-DNA杂交链（RNA-DNA hybrid）的定位进行了绘制。雌二醇是一种强效转录激活因子，可诱导激素饥饿状态下的细胞增殖。本研究发现，在已知受雌激素受体调控的基因区域中，RNA-DNA杂交链的水平出现显著上调。本研究还对经两种不同雌二醇处理时间点以及空白对照（mock control）处理的MCF7细胞内的RNA:DNA杂交链水平进行了检测。