Herbs, ingredients and targets of SWT and SJZT1.rar

<b>Traditional Chinese Medicine (TCM) is increasingly getting clinical application worldwide. But its theory like QI-Blood is still abstract. Actually, Qi deficiency and blood deficiency, which were treated by Si-Jun-Zi-Tang (SJZT) and Si-Wu-Tang (SWT) respectively, have characteristic clinical manifestations. Here, we analyzed targets of the ingredients in SJZT and SWT to unveil potential biologic mechanisms between Qi deficiency and blood deficiency through biomedical approaches. First, ingredients in SWT and SJZT were retrieved from TCMID database. The genes targeted by these ingredients were chosen from STITCH. After enrichment analysis by Gene Ontology (GO) and DAVID, enriched GO terms with p-value less than 0.01 were collected and interpreted through DAVID and KEGG. Then a visualized network was constructed with ClueGO. Finally, a total of 243 genes targeted by 195 ingredients of SWT formula and 209 genes targeted by 61 ingredients of SJZT were obtained. Six </b> <b>metabolism pathways and two environmental information processing pathways </b><b>enriched by targets were correlated with two or more herbs in SWT and SJZT formula, respectively. SWT significantly influence amino acid and carbohydrate metabolism. While SJZT mainly influence the organismal system function, environmental information processing, metabolism and cellular processes.</b>中医药（Traditional Chinese Medicine, TCM）在全球范围内的临床应用日益广泛，但其气血理论仍较为抽象。事实上，气虚与血虚分别可通过四君子汤（Si-Jun-Zi-Tang, SJZT）与四物汤（Si-Wu-Tang, SWT）进行干预，二者均具有特征性的临床表现。本研究通过生物医学手段分析四君子汤与四物汤所含成分的作用靶点，以揭示气虚与血虚潜在的生物学机制。首先，从TCMID数据库中检索四物汤与四君子汤的活性成分；随后从STITCH数据库中获取这些成分所靶向的基因。通过基因本体论（Gene Ontology, GO）与DAVID进行富集分析后，收集p值小于0.01的显著富集GO条目，并通过DAVID与京都基因与基因组百科全书（KEGG）进行注释解读；随后使用ClueGO构建可视化调控网络。最终共获得四物汤195种成分所靶向的243个基因，以及四君子汤61种成分所靶向的209个基因。上述靶点共富集得到6条代谢通路与2条环境信息处理通路，分别与四物汤及四君子汤中的2种及以上药材相关。四物汤可显著影响氨基酸与碳水化合物代谢；而四君子汤则主要作用于机体系统功能、环境信息处理、代谢及细胞过程。