809094 Biomarker Identification in Fracture Healing (experiment #3808 miRNA)

The specific genes that distinguish normal fracture healing from abnormal healing or nonunion in humans are unknown. This study explored global RNA expression in remnant bone fragments from acutely injured patients undergoing open primary fracture repair surgery. These data will be compared to blood samples collected from acutely injured subjects, chronic nonunion subjects, and a panel of healthy volunteers. We used microarrays to do a global comparison across bone fragments collected from different patients, and different bones. We also evaluated the role of remnant bone preparation which was either immediate freezing in liquid nitrogen or heat stabilized with the Denator Stabilizer(TM) system. Remnant bone fragments were collected intraoperatively by the staff of the Cooperative Human Tissue Network Eastern Division (CHTN-ED https://www.chtneast.org/). Samples were split for processing as frozen in liquid nitrogen vs. heat stabilization, followed by annotation and storage until the time of RNA extraction.

目前尚不明确区分人类正常骨折愈合与异常愈合或骨不连的特异性基因。本研究针对接受开放性一期骨折修复手术的急性创伤患者的残余骨碎片，开展了全转录组RNA表达谱分析。后续将把该全转录组RNA表达数据集与急性创伤受试者、慢性骨不连受试者以及健康志愿者队列的血液样本数据进行比对分析。本研究利用基因芯片技术，对不同患者、不同骨骼来源的残余骨碎片开展全转录组表达比对分析。同时，本研究还评估了残余骨标本的两种制备流程对实验的影响——即即刻液氮冻存与使用Denator Stabilizer(TM)系统进行热稳定处理。残余骨碎片由东部协作人体组织网络（Cooperative Human Tissue Network Eastern Division, CHTN-ED，https://www.chtneast.org/）的工作人员在术中采集。采集后的样本被分为两组，分别采用液氮冻存与热稳定处理流程进行制备，随后进行样本注释与低温保存，直至后续开展RNA提取操作。