Genome-wide identification of target genes for the transcription factor Sox10 in the Msc80 Schwann cell line.

To identify target genes of the Sox10 transcription factor in peripheral glia in a genome-wide and unbiased approach, we performed CRISPR/Cas9-dependent genome editing of the mouse Sox10 gene in the Msc80 Schwann cell line, followed by RNA-Seq studies on Sox10-expressing control Msc80 cells and Sox10-negative genome edited cell clones. Among others we detected substantial changes in the expression of genes associated with Schwann cell adhesion and the formation of the node of Ranvier. Overall design: We generated mRNA profiles of Msc80 cells (ctrl) and Sox10-negative Msc80 cells (Sox10ko) using the Illumina HiSeq 2500 platform.

为了以全基因组无偏的方式鉴定外周胶质细胞中Sox10转录因子的靶基因，我们在Msc80雪旺细胞系中对小鼠Sox10基因开展了CRISPR/Cas9依赖的基因组编辑，随后对表达Sox10的对照Msc80细胞以及Sox10阴性的基因组编辑细胞克隆进行了RNA测序（RNA-Seq）分析。我们检测到，除其他变化外，与雪旺细胞黏附及郎飞结（node of Ranvier）形成相关的基因表达出现了显著改变。
总体实验设计：我们采用Illumina HiSeq 2500平台，构建了Msc80细胞（对照组，ctrl）与Sox10敲除Msc80细胞（Sox10ko）的mRNA表达谱。