SETMAR regulates gene expression and alternative splicing. SETMAR regulates gene expression and alternative splicing

Transcriptomic profiling of WT and SETMAR KO HEK293T cells. Overall design: To determine how SETMAR impacts the transcriptome, CRISPR/Cas9 was used to generate HEK293T SETMAR KO cells. RNAseq was performed on two KO clones and WT cells, four replicates for each. Differential expression analysis revealed 163 genes with altered gene expression in both clones. Alternative splicing analysis revealed 255 shared alternative splicing events. Collectively, our results support a model in which SETMAR likely impacts AS through TIR-specific DNA-binding and lysine methyltransferase activities, consistent with a role in development of simian primates.

野生型（Wild Type, WT）与SETMAR敲除（Knockout, KO）HEK293T细胞的转录组谱分析。实验设计概述：为探究SETMAR对细胞转录组的调控功能，本研究利用CRISPR/Cas9技术构建HEK293T细胞的SETMAR敲除模型。分别对2株敲除克隆株与野生型细胞进行RNA测序（RNA-seq），每组设置4个生物学重复。差异表达分析结果显示，两株敲除克隆中共鉴定出163个差异表达基因；可变剪接（Alternative Splicing, AS）分析则发现255个两株克隆共有的可变剪接事件。综上，本研究结果支持如下模型：SETMAR可能通过其TIR特异性DNA结合活性与赖氨酸甲基转移酶活性调控可变剪接，该发现与SETMAR在类人猿发育过程中所发挥的功能相一致。