Statistical evaluation of the chromatin structural analysis.

Statistical evaluation of the differences in chromatin structure after targeting EGFP-lacR tagged constructs. Rows 1 through 7 show the comparison between control cells transfected with EGFP-lacR and cells transfected with EGFP-lacR-tagged full-length MeCP2, EGFP-lacR-tagged VP16 and EGFP-lacR-tagged separate MeCP2 domains (i.e. C-terminus, MBD, TRD, ΔC-terminus, R133C Rett syndrome mutation). Since the data are not normally distributed and do not have a shared variance, we used Wilcoxon nonparametric statistical testing corrected for multiple testing (Bonferoni) [48]. The p-values are shown, indicating the probabilities that two populations are different choosing a cut-off value of p = 0.007. Based on this analysis the EGFP-lacR tagged VP16, MeCP2 (full length) and the R133C population are significantly different from EGFP-lacR control, whereas EGFP-lacR tagged MBD, TRD, C-terminus and ΔC-terminus, are not significantly different from EGFP-lacR.