Statistical evaluation of the chromatin structural analysis.

Statistical evaluation of the differences in chromatin structure after targeting EGFP-lacR tagged constructs. Rows 1 through 7 show the comparison between control cells transfected with EGFP-lacR and cells transfected with EGFP-lacR-tagged full-length MeCP2, EGFP-lacR-tagged VP16 and EGFP-lacR-tagged separate MeCP2 domains (i.e. C-terminus, MBD, TRD, ΔC-terminus, R133C Rett syndrome mutation). Since the data are not normally distributed and do not have a shared variance, we used Wilcoxon nonparametric statistical testing corrected for multiple testing (Bonferoni) [48]. The p-values are shown, indicating the probabilities that two populations are different choosing a cut-off value of p = 0.007. Based on this analysis the EGFP-lacR tagged VP16, MeCP2 (full length) and the R133C population are significantly different from EGFP-lacR control, whereas EGFP-lacR tagged MBD, TRD, C-terminus and ΔC-terminus, are not significantly different from EGFP-lacR.针对靶向标记有EGFP-lacR的构建体后染色质结构的差异展开统计学评估。第1至7行展示了转染EGFP-lacR的对照细胞，与转染EGFP-lacR标记的全长MeCP2、EGFP-lacR标记的VP16以及EGFP-lacR标记的独立MeCP2结构域（即C端、MBD、TRD、ΔC端、R133C雷特综合征突变体）的细胞之间的比较。由于数据不满足正态分布且方差不齐，我们采用了经邦费罗尼多重检验校正（Bonferoni）的威尔科克森非参数统计检验（Wilcoxon nonparametric statistical testing）[48]。本次检验给出了p值，其代表两个总体存在差异的概率，设定的显著性截断值为p=0.007。基于该分析结果，EGFP-lacR标记的VP16、全长MeCP2以及R133C突变体细胞群，与EGFP-lacR对照细胞存在显著差异；而EGFP-lacR标记的MBD、TRD、C端以及ΔC端结构域组，则与EGFP-lacR对照细胞无显著差异。