Promoter proximal pausing and its regulation by c-Myc in embryonic stem cells: ChIP-Seq

Recruitment of the RNA Polymerase II (Pol II) transcription initiation apparatus to promoters by specific DNA binding transcription factors is well recognized as a key regulatory step in gene expression. We describe here evidence that promoter-proximal pausing is a general feature of transcription by Pol II in embryonic stem (ES) cells, and thus an additional step where regulation of gene expression may occur. We report here that c-Myc, which occupies a third of actively transcribed genes in ES cells and is a key regulator of cellular proliferation, binds P-TEFb and contributes to release of promoter-proximal paused Pol II at these genes. ChIP-seq data for Pol II and additional factors controlling pause release in mouse ES cells.

特异性DNA结合转录因子将RNA聚合酶II（RNA Polymerase II，Pol II）转录起始复合物招募至启动子区域，这一过程已被公认为基因表达调控的关键步骤。本文阐述相关证据表明，启动子近端暂停是胚胎干细胞（embryonic stem cells，ES细胞）中RNA聚合酶II转录的普遍特征，因此这也是基因表达调控可能发生的又一环节。我们在此报告：在小鼠胚胎干细胞中，占据三分之一活跃转录基因、且作为细胞增殖关键调控因子的c-Myc可结合正性转录延伸因子b（P-TEFb），并促进上述基因位点处启动子近端暂停的Pol II的释放。本数据集包含小鼠胚胎干细胞中Pol II及调控转录暂停释放的其他相关因子的染色质免疫共沉淀测序（ChIP-seq）数据。