Table_7_Proteomics-Based Data Integration of Wheat Cultivars Facing Fusarium graminearum Strains Revealed a Core-Responsive Pattern Controlling Fusarium Head Blight.XLSX

Fusarium head blight (FHB), mainly occurring upon Fusarium graminearum infection in a wide variety of small-grain cereals, is supposed to be controlled by a range of processes diverted by the fungal pathogen, the so-called susceptibility factors. As a mean to provide relevant information about the molecular events involved in FHB susceptibility in bread wheat, we studied an extensive proteome of more than 7,900 identified wheat proteins in three cultivars of contrasting susceptibilities during their interaction with three F. graminearum strains of different aggressiveness. No cultivar-specific proteins discriminated the three wheat genotypes, demonstrating the establishment of a core proteome regardless of unequivocal FHB susceptibility differences. Quantitative protein analysis revealed that most of the FHB-induced molecular adjustments were shared by wheat cultivars and occurred independently of the F. graminearum strain aggressiveness. Although subtle abundance changes evidenced genotype-dependent responses to FHB, cultivar distinction was found to be mainly due to basal abundance differences, especially regarding the chloroplast functions. Integrating these data with previous proteome mapping of the three F. graminearum strains facing the three same wheat cultivars, we demonstrated strong correlations between the wheat protein abundance changes and the adjustments of fungal proteins supposed to interfere with host molecular functions. Together, these results provide a resourceful dataset that expands our understanding of the specific molecular events taking place during the wheat–F. graminearum interaction.

禾谷镰孢菌（Fusarium graminearum）侵染多种小粒谷类作物引发的赤霉病（Fusarium head blight, FHB），其致病过程被认为依赖于该真菌病原菌操控的一系列宿主过程，即所谓的感病因子（susceptibility factors）。为解析普通小麦（bread wheat）中赤霉病感病相关的分子事件，本研究针对三个抗性差异显著的小麦品种，在其与三株致病力各异的禾谷镰孢菌菌株互作过程中，对包含7900余个已鉴定小麦蛋白的广泛蛋白质组（proteome）进行了分析。未检测到可区分这三个小麦基因型的品种特异性蛋白，表明即便赤霉病感病性存在显著差异，小麦仍会形成一套核心蛋白质组。蛋白质定量分析结果显示，多数由赤霉病诱导的分子调控事件在小麦品种间保守存在，且不受禾谷镰孢菌菌株致病力的影响。尽管蛋白丰度的细微变化体现了不同基因型对赤霉病的特异性应答，但品种间的差异主要源于基础丰度的差异，这一点在叶绿体功能相关蛋白中尤为突出。将本研究数据与此前针对三株禾谷镰孢菌菌株与上述三个小麦品种互作的蛋白质组图谱数据进行整合后，我们发现小麦蛋白丰度变化与本应干扰宿主分子功能的真菌蛋白调控过程之间存在显著相关性。综上，本研究所得的丰富数据集，可进一步拓展我们对小麦与禾谷镰孢菌互作过程中特定分子事件的认知。