Quantitative analysis of WT, Dnmt3a-/-, Dnmt3b-/- and Tet1-/- J1 ES cell transcriptomes. Mus musculus

In order to figure out the roles of Dnmt3 and Tet1 in gene regulation, we assessed global gene expression changes in Dnmt3aKO, Dnmt3bKO and Tet1KO ES cells. Overall design: mRNA profiles of WT, Dnmt3a-/- and Dnmt3b-/- ES cells; WT and Tet1-/- ES cells were generated by deep sequencing, in replicate, using Illumina HiSeq sequencing systems.

为明确DNA甲基转移酶3（Dnmt3）与Tet1在基因调控中的功能，我们对Dnmt3a敲除（Dnmt3aKO）、Dnmt3b敲除（Dnmt3bKO）及Tet1敲除（Tet1KO）胚胎干细胞（ES cells）中的全基因组基因表达变化开展了分析评估。
实验整体设计：采用Illumina HiSeq测序系统进行深度测序并设置生物学重复，获取野生型（WT）、Dnmt3a-/-、Dnmt3b-/-胚胎干细胞，以及野生型（WT）、Tet1-/-胚胎干细胞的mRNA表达谱。