Non-Invasive Biomarkers for Diagnosing and Anticipating Post-Transplant Complications

In this study, we investigated the role of the gut microbiota on the development of complications in kidney transplant recipients. We collected serial fecal specimens from 168 kidney transplant recipients within the first 3 months after transplantation. We performed 16S rRNA gene sequencing of the V4-V5 hypervariable region and examined whether the relative gut abundance of pathogenic bacteria was associated with future development of complications like bacteriuria and urinary tract infections. In a subset of samples, we performed metagenomic sequencing of stool and urine supernatant specimens to determine strain level analysis. We collected an additional 5 fecal specimens from 5 kidney transplant recipients to evaluate the gut microbiota's ability to convert inactive mycophenolic acid... (for more see dbGaP study page.)

本研究探究了肠道菌群在肾移植受者并发症发生发展中的作用。我们于肾移植术后前3个月内，收集了168名肾移植受者的系列粪便标本。针对V4-V5高变区，我们开展了16S核糖体RNA（16S rRNA）基因测序，分析病原菌的肠道相对丰度是否与菌尿、尿路感染等并发症的后续发生存在关联。我们还对部分样本的粪便及尿液上清标本进行宏基因组测序，以开展菌株水平分析。此外，我们从5名肾移植受者处额外收集了5份粪便标本，以评估肠道菌群转化非活性麦考酚酸的能力……（更多详情请参见dbGaP研究页面）