Equal parental contribution to the transcriptome is not equal control of embryogenesis

The goal of present study is to investigate parental contributions to the transcriptome of apical and basal cell lineages in early embryogenesis. We found equivalent parental genome derived reads in the transcriptome of apical and basal cell lineages of early proembryos. Hundreds of parent-of-origin genes were identified in both apical and basal cell lineages according to our allele- specific transcriptome data. Overall design: To determine parental contributions to apical and basal cell lineages, the transcriptome of apical and basal cell lineages of early proembryos at 1-cell and 32-cell embryo stages derived from the crosses between the Col-0 and Ler were sequenced. To minimize the variance and avoid the influence of inherent allele-specific expression in the transcriptome, three independent biological replicates were performed for each hybrid cell type.

本研究旨在探究早期胚胎发生过程中，亲本对顶端与基部细胞谱系转录组的贡献。我们在早期原胚的顶端与基部细胞谱系转录组中，发现来源于亲本基因组的测序读段占比相当。基于等位基因特异性转录组数据，我们在顶端与基部两类细胞谱系中均鉴定出了数百个亲本来源基因。总体实验设计：为明确亲本对顶端与基部细胞谱系的贡献，我们对源自Col-0与Ler杂交的1细胞期及32细胞期早期原胚的顶端与基部细胞谱系转录组进行了测序。为最小化实验误差并避免转录组中固有等位基因特异性表达的干扰，我们为每一类杂交来源的细胞谱系设置了三次独立生物学重复。