CHD1 in yeast is recruited by transcription elongation factors and maintains H3K4me3/H3K36me3 domains at actively transcribed and spliced genes [ChIP-seq]. Saccharomyces cerevisiae

We report genome-wide Chd1 occupancy in WT and in ten elongation factor mutants, and genome-wide H3K4me3/H3K36me3 profiles in WT and CHD1 mutant. Overall design: ChIP-seq for Chd1 in wild-type and in ten elongation factor mutants. ChIP-seq for H3K4me3/H3K36me3 in wild-type and CHD1 mutant.

本研究报道了野生型（wild-type, WT）及10种延伸因子突变体中全基因组范围的Chd1（Chromodomain Helicase DNA Binding Protein 1）结合占有率，以及野生型与CHD1突变体中全基因组范围内的H3K4me3/H3K36me3修饰谱。
实验设计：针对野生型及10种延伸因子突变体开展Chd1的染色质免疫共沉淀测序（Chromatin Immunoprecipitation Sequencing, ChIP-seq）；针对野生型与CHD1突变体开展H3K4me3/H3K36me3的染色质免疫共沉淀测序。