Assessment of non-invasive tear break-up time and tear meniscus height after instillation of three different formulations of anesthetic eye drops by Oculus Keratograph 5M

Abstract Purpose: To assess the non-invasive tear break-up time (NITBUT) and tear meniscus height (TMH) after instilling the three different types of anesthetic eye drops by Oculus Keratograph 5M. Methods: In this prospective study, 85 healthy subjects (85 eyes) were randomly divided into three groups. The groups were randomly received lidocaine hydrochloride 2%, proparacaine hydrochloride 0.5%, and tetracaine hydrochloride 0.5%. The qualitative and quantitative parameters of tear film were assessed using NITBUT and TMH, respectively. In all groups, the quantity of tear film using TMH was measured in the right eye of subjects, while the quality of tear film using NITBUT was assessed in the left eye. The analysis of variance (ANOVA) was used to compare the difference between before and after the intervention. A P-value < 0.05 was considered significant. Results: Differences for TMH and NITBUT between before and after applying lidocaine hydrochloride 2% were not statistically significant (P > 0.05). The mean values of NITBUT and TMH after the instillation of proparacaine hydrochloride 0.5% showed a significant decrease than before the intervention (P < 0.05). Also, after the use of tetracaine hydrochloride 0.5%, the mean value of NITBUT was significantly increased (P < 0.05), but the mean value of TMH was significantly decreased than before the intervention (P < 0.05). Conclusion: Our study showed that lidocaine hydrochloride 2% as an anesthetic eye drops can be an appropriate choice for eye examinations due to a lack of significant effect on the quantity and quality of tear film.

摘要：
目的：采用Oculus Keratograph 5M眼表检测仪，评估三种不同类型麻醉滴眼液滴用后，受试者的非侵入性泪膜破裂时间（non-invasive tear break-up time, NITBUT）与泪河高度（tear meniscus height, TMH）。
方法：本前瞻性研究共纳入85名健康受试者（共85只眼），按随机原则分为三组。三组受试者分别滴用2%盐酸利多卡因、0.5%盐酸丙美卡因及0.5%盐酸丁卡因。分别采用非侵入性泪膜破裂时间（NITBUT）与泪河高度（TMH）评估泪膜的定性与定量参数。所有组均通过测量受试者右眼的泪河高度以获取泪膜量值，通过左眼的非侵入性泪膜破裂时间评估泪膜质量。采用方差分析（analysis of variance, ANOVA）比较干预前后的差异，以P值<0.05为差异具有统计学意义。
结果：滴用2%盐酸利多卡因后，受试者的泪河高度（TMH）与非侵入性泪膜破裂时间（NITBUT）干预前后差异均无统计学意义（P>0.05）。滴用0.5%盐酸丙美卡因后，受试者的NITBUT与TMH均值均较干预前显著降低（P<0.05）。滴用0.5%盐酸丁卡因后，受试者的NITBUT均值显著升高（P<0.05），但TMH均值较干预前显著降低（P<0.05）。
结论：本研究显示，2%盐酸利多卡因作为麻醉滴眼液，对泪膜的质与量均无显著影响，因此可作为眼部检查的合适选择。