Experimental and predicted ∆V_1/2 of L235 and V236 mutations.

The four novel mutations are bolded. >150 signifies the case where the complete G-V curve could not be measured due to extremely low activity of the mutant. The WT “mutations”, L235L and V236V, reflect the inherent uncertainty of the RF model prediction. “Previously acquired” denotes previously acquired data from the Cui lab that were available prior to model training. *The experimental result for L235A was published after model training, so this mutation is also a true test case.四种新型突变以粗体标注。>150代表因突变体活性极低而无法测定完整G-V曲线（G-V curve）的情况。野生型（Wild Type, WT）"突变"（即L235L与V236V）体现了随机森林（Random Forest, RF）模型预测的固有不确定性。"预获取数据"指模型训练前已由崔实验室（Cui lab）获取的既往实验数据。*L235A的实验结果在模型训练完成后才正式发表，因此该突变同样属于真实测试样本。