Bulk RNA-seq of RSC96 Schwann cells after magnetic nanoparticle uptake and magnetic force stimulation

To explore the effect of magnetic mechanical forces mediated by magnetic nanoparticles on the repair phenotype of Schwann cells in a gradient magnetic field environment, we co-cultured magnetic nanoparticles with RSC96 cells. Overall design: The magnetic nanoparticles were taken up by the cells, magnetizing the RSC96 cells, and the magnetized cells were exposed to a gradient magnetic field environment and subjected to magnetic force stimulation. Normal cultured control group cells and experimental group cells that received magnetic force stimulation were collected for mRNA transcriptome sequencing to observe the effect of magnetic nanoparticle-mediated magnetic force stimulation on the gene expression profile of Schwann cells.

为探究梯度磁场环境中，磁性纳米颗粒（magnetic nanoparticles）介导的磁机械力对施万细胞（Schwann cells）修复表型的影响，本研究将磁性纳米颗粒与RSC96细胞进行共培养。实验整体设计如下：将磁性纳米颗粒加入细胞体系使其被细胞摄取，使RSC96细胞获得磁化；随后将磁化后的RSC96细胞置于梯度磁场环境中，施加磁机械力刺激。分别收集常规培养的对照组细胞与接受磁机械力刺激的实验组细胞，开展mRNA转录组测序，以分析磁性纳米颗粒介导的磁机械力刺激对施万细胞基因表达谱的影响。