The rat-tail intervertbral disc cells was incubated with NGF at a concentration of 100 ng/ml/day for a total of five days. Primary culture of 8 weeks rat-tail interverbral disc cells. The control grou
During embryonic development, the notochord is the precursor to the nucleus pulposus (NP) in the intervertebral disc (IVD), where the notochord cells differentiate to become notochordal-like cells in
Genomic DNA, isolated from 24 human intervertebral disc tissue, enriched for bacterial DNA with NEB® microbiome kit, amplified using 16SrRNA specific primers (V1-V9) and sequenced using Illumina MiSeq