RNA-seq of mouse pancreatic pericytes from wild-type and Tcf7l2-mutated mice

To characterise their gene expression, wild type pancreatic pericytes were isolated based on the YFP-labeling from Nkx3.2-Cre;EYFP mice. As control, whole isolated islets were analysed.To identify Tcf7l2-depenedent genes, pancreatic pericytes were isolated from Nkx3.2-Cre;EYFP;Tcf7l2 flox/flox transgenic mice, and compared to wild type cells.

为表征胰腺周细胞的基因表达特征，研究人员基于YFP标记（Yellow Fluorescent Protein）从Nkx3.2-Cre;EYFP转基因小鼠中分离得到野生型胰腺周细胞，并以分离得到的完整胰岛作为对照样本进行分析。为鉴定Tcf7l2（T-cell factor 7-like 2）依赖性基因，研究人员从Nkx3.2-Cre;EYFP;Tcf7l2 flox/flox转基因小鼠中分离胰腺周细胞，并将其与野生型细胞进行对照比较。